tedu

High pressure, high forces, long lever arms...all of that meant heavy and strong (read: expensive) parts which I was not looking forward to having to fabricate. Instead, I settled on the simpler idea of harnessing the power of compressed gas. Instead of using a high mechanical advantage lever to push a piston, compressed CO2 would be dispensed from a small and inexpensive 12g or 16g cartridge which would then generate the requisite pressure to properly extract espresso. This concept is not actually novel; both an unsuccessful kickstarter and a now-defunct handheld espresso maker (with a fanatical user base) employed this mechanism.

Espresso coffee is among the most consumed beverages in the world. Recent studies report a protective activity of the coffee beverage against neurodegenerative disorders such as Alzheimer′s disease. Alzheimer′s disease belongs to a group of disorders, called tauopathies, which are characterized by the intraneuronal accumulation of the microtubule-associated protein tau in fibrillar aggregates. In this work, we characterized by NMR the molecular composition of the espresso coffee extract and identified its main components. We then demonstrated with in vitro and in cell experiments that the whole coffee extract, caffeine, and genistein have biological properties in preventing aggregation, condensation, and seeding activity of the repeat region of tau. We also identified a set of coffee compounds capable of binding to preformed tau fibrils. These results add insights into the neuroprotective potential of espresso coffee and suggest candidate molecular scaffolds for designing therapies targeting monomeric or fibrillized forms of tau.

In vitro results, take with a grain of salt or shot of espresso.